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This conference is managed by the American Society for Microbiology

Learning Objectives

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Course level

 1.  To master the core concepts in molecular biology, genetics, and cell biology. (Foundational knowledge)

2.  Acquire basic proficiency in common lab techniques in cell and molecular biology.  (Application)

3.  Become competent in reading and writing scientific literature. (Learning how to learn)

4.  Begin to see themselves as creators, not just consumers, of scientific knowledge.  (Learning how to learn/Caring)

5.  Learn to be resilient in the face of uncertainty and setbacks. (Human dimension)

Analysis of RBC membrane proteins

This is the first of 3 lab projects in a 1 semester introductory cell and molecular lab course for pre-med majors.    

In the project students isolate RBC membrane proteins from whole mouse blood and compare protein profiles between normal mouse blood and a spherocytotic mutant.  (Mutant obtained from collaborator at Medical College of Wisconsin.)  

Current summative assessment includes an abbreviated lab report written in scientific manuscript style and MC and calculation/short answer questions on a final exam.  Formative assessment includes peer review of lab report, and in class problems sets.

At the end of this module, students will be able to:

Foundational knowledge:

•    Diagram, label and explain how SDS-PAGE separates proteins by size.

•    Compare Coomasie stained gel with a Western blot.
•    Recognize the 4 major sections of a scientific publication.

•    Interpret the results of SDS-PAGE electrophoresis.
•    Communicate the results of an experiment in a professional scientific format.

Learning how to learn:
•    Connect an experiment to the broader field of study.


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