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Sample Assessment Questions

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Sample assessment questions for CHEM347:


Learning Objectives:           Students will list and describe the 4 levels of protein structure.

                                       Students will describe Prion diseases and the relationship to protein folding


Blooms Category:  Analysis and Evaluate


Given what you know about levels of protein structure:

a.        Distinquish between the structures of PrPc and PrPsc

b.       Explain the mechanism responsible for the differences in shape

c.        Predict what would happen to the PrPc structure if  the mutation in Cruetfeld-Jakob Disease were at ValàLys

This is a summative question for an exam


Learning Objective:      Students will list and describe methods of protein purification

                                Students will recognize protein characteristics and select the correct method of



Blooms Category:  Knowledge, Comprehension, and Analysis (Synthesis)


A.      A.  List and describe the 3 methods of protein purification we have performed in laboratory.

B.      B.  Which method would be most effective for purification of the EBV thymidine kinase protein? (TK is a     95kD, highly negative protein)

C     C. Propose an experimental protocol you could set up to purify EBV TK using your chosen method.

 This is a formative in class group activity.


Learning Objective:       Students will retrieve gene and protein sequences from a database and perform a    series ofBlast searches


Blooms Category:  Application, Analysis and Evaluation

You have been assigned the following Drosophila melanogaster, SWA1 (swallow).  Using Flybase, Genbank, NCBI blast, and SwissPro and Genescan complete the following:

a.       Retrieve the genomic sequence as a FASTA file (store in Sequence Folder on the Wiki)

b.       Using NCBI blast perform a tblastx search on your gene sequence.  Capture and store on the Wiki.

c.        Determine the top ten hits based on E value.  Report these on your data sheet.

d.       Determine the first related species not in Drosophila.  Report this on your data sheet.

e.        Using SwissPro analyze the protein sequence of your gene for functional domains, binding motifs, structural features.  Report your findings on your data sheet.

f.        Determine secondary structural features of your protein.

g.        Using Genescan, predict the genomic structure of your gene. Include all exons, introns and splice sites in the model.  Paste link up on the Wiki.

 In 1-2 pages report the putative function of your protein based on your interpretation of your results from the above modeling programs.  Include potential irregularities in your predictions.

This would be an in class and then homework writing assignment.


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